Differential expression spectrum and targeted gene prediction of tRNA-derived small RNAs in idiopathic pulmonary arterial hypertension

Background: Idiopathic pulmonary arterial hypertension (PAH) is a potentially fatal vascular disease with an extremely poor natural course. The limitations of current treatment and the unclear etiology pathogenesis idiopathic PAH require new targets avenues exploration involved in PAH. tRNA-derived small RNAs (tsRNAs), type non-coding RNAs, have significant part progress diverse diseases. However, potential functions behind tsRNAs remain unknown. Methods: Small RNA microarray was implemented on three pairs plasma patients healthy controls to investigate compare expression profiles. Validation samples were used for real-time polymerase chain reaction (Real-time PCR) verify several dysregulated tsRNAs. Bioinformatic analysis adopted determine target genes mechanisms validated Results: Microarray detected 816 statistically significantly tsRNAs, which 243 upregulated 573 downregulated Eight results Real-time PCR concordant microarray: four (tRF3a-AspGTC-9, 5’tiRNA-31-GluCTC-16, i-tRF-31:54-Val-CAC-1 tRF3b-TyrGTA-4) (5’tiRNA-33-LysTTT-4, i-tRF-8:32-Val-AAC-2, i-tRF-2:30-His-GTG-1, i-tRF-15:31-Lys-CTT-1). Gene Ontology has shown that verified are related cellular macromolecule metabolic process, regulation process. It disclosed widely pathways by Kyoto Encyclopedia Genes Genomes. Conclusion: This study investigated tsRNA profiles found may become novel biomarkers possible